The ion of phosphotungstic acid contains 12 tungsten atoms, and its chemical structure was not very clear in the early days. It is also called phosphorus-24-tungstic acid, and its chemical formula is 3H2O•P2O5 24WO3.59H2O, (P2W24O80H6)•29H2O, in which tungsten, phosphorus The amount of oxygen and oxygen is wrong, but the ratio is correct. It was not until the 1970s that the correct chemical formula was slowly determined. In histology, phosphotungstic acid is commonly used in the staining of cell samples, and it is often used together with hematoxylin, which is called phosphotungstic acid-hematoxylin staining. Phosphotungstic acid will combine with fibrin, collagen and fibers in connective tissue to form different colors. Phosphotungstic acid is an electron-dense substance that is opaque under electrons. In transmission electron microscopy, phosphotungstic acid can be used to negatively stain viruses, nerves, polysaccharides and other biological tissues.
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