Yes, tungsten needles can be used for microscale patterning of surfaces for cell culture. Tungsten needles can be used to create microscale patterns on a variety of substrates, including glass, silicon, and polydimethylsiloxane (PDMS). These patterns can be used to create microenvironments for cells to grow and differentiate. For example, microscale patterns can be used to control the orientation and alignment of cells, as well as the stiffness of the substrate. Tungsten needles are often used in combination with microfabrication techniques, such as soft lithography and microcontact printing, to create these microscale patterns.